| EST name | EST sequence |
| gi|74532215|gb|DT741066.1|DT741066 | GATGATTCTCCAGTACCCTAAACTCTCTTCTTCTCAAAAAATTAACAATGGCTTCAATGACTACTGCTTTCACTTCTCAT CTTTCTTCCAAAATCAAAACCCCTTTCTTAGATAACCAATCATCTTTCCATGTCACTCCATCTCGCATCCAACTCACTCG TTCATCTTCCCCAAACACTCACATCACCATGTCTTCATCATCATCATCATCATCTTCATCTTATGATCTATCTTCCTTCA AATTTGAACCAATTAAGGAATCCATTGTATCACGTGAGATGACTCGCAGATACATGATGGATATGATTACATATGCTGAC ACTGATGTTGTTGTTGTTGGAGCAGGTTCAGCAGGTCTTTCTTGTGCTTATGAAATGAGCAAAAACCCTAACATCAACAT CGCTATTATCGAACAATCTGTCAGTCCTGGTGGTGGTGCTTGGTTAGGTGGTCAGCTTTTTTCTGCCATGGTTGTTCGCA AACCTGCTCACATCTTCCTTGATGAACTCGGTGTTGAATACGATGAACAAGACACCTATGTTGTTATCAAGCACGCTGCC TTGTTTACATCCACAATCATGTCTAAGCTTTTGGCTAAACCAAATGTGAAACTTTTTAATGCTGTTGCTGCTGAGGATTT GATCGTGAAAGAAGGTAAAGTTGCTGGGGTTGTCACAAATTGGGCTTTGGTTTCTATGAATCATGATACACAATCATGTA TGGATCCAAATGTAATGGAAGCTAAGATTGTTGTCAGTTCTTGTGGCCATGATGGTCCTTTTGGTGCTACTGGTGTGAGA GGCTGAGAGCATTGGAATGATTGATTCTGTTCCTGGTATGAAAGCTCTTGATATGATACTGCTGAGATGCTATTGTCAGA TGACAGAGAATTG |
Most similar to sp|O23787|THI4_CITSI
>BLASTX 2.2.20 [Feb-08-2009]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
>sp|O23787|THI4_CITSI Thiazole biosynthetic enzyme, chloroplastic
OS=Citrus sinensis GN=THI1 PE=2 SV=1
Length = 356
Score = 350 bits (899), Expect(2) = 3e-98
Identities = 187/253 (73%), Positives = 198/253 (78%), Gaps = 2/253 (0%)
Frame = +3
Query: 48 MASM-TTAFTSHLSSKIKTPFLDNQSSFHVTPSRIQLTRXXXXXXXXXXXXXXXXXXXXX 224
MA+M +TAF +SS F SSFH P L R
Sbjct: 1 MAAMASTAFAPSVSSTTNKLF---DSSFHGAPMSPSLLRLQPIKSSRPNNLSISASASPP 57
Query: 225 XXXX-FKFEPIKESIVSREMTRRYMMDMITYADTDXXXXGAGSAGLSCAYEMSKNPNINI 401
FKF+PIKESIVSREMTRRYM DMITYADTD GAGSAGLSCAYE+SKNPNI I
Sbjct: 58 YDLNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQI 117
Query: 402 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGVEYDEQDTYVVIKHAALFTSTIM 581
AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELG++YDEQD YVVIKHAALFTSTIM
Sbjct: 118 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIM 177
Query: 582 SKLLAKPNVKLFNAVAAEDLIVKEGKVAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSS 761
SKLLA+PNVKLFNAVAAEDLIVK G+V GVVTNWALVSMNHDTQSCMDPNVMEAK+VVSS
Sbjct: 178 SKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 237
Query: 762 CGHDGPFGATGVR 800
CGHDGPFGATGV+
Sbjct: 238 CGHDGPFGATGVK 250
Score = 30.4 bits (67), Expect(2) = 3e-98
Identities = 13/15 (86%), Positives = 14/15 (93%)
Frame = +1
Query: 811 IGMIDSVPGMKALDM 855
IGMI+ VPGMKALDM
Sbjct: 255 IGMIEEVPGMKALDM 269
>sp|Q38709|THI4_ALNGL Thiazole biosynthetic enzyme, chloroplastic
OS=Alnus glutinosa GN=THI4 PE=2 SV=1
Length = 352
Score = 335 bits (860), Expect(2) = 2e-94
Identities = 164/188 (87%), Positives = 176/188 (93%)
Frame = +3
Query: 237 FKFEPIKESIVSREMTRRYMMDMITYADTDXXXXGAGSAGLSCAYEMSKNPNINIAIIEQ 416
F F+PIKESIVSREMTRRYMMDMITYADTD GAGS+GL C YE+SKNP++ +AIIEQ
Sbjct: 60 FTFDPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSSGLVC-YELSKNPSVQVAIIEQ 118
Query: 417 SVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGVEYDEQDTYVVIKHAALFTSTIMSKLLA 596
SVSPGGGAWLGGQLFS MVVRKPAH+FLDELG+EYDEQD YVVIKHAALFTSTIMSKLLA
Sbjct: 119 SVSPGGGAWLGGQLFSGMVVRKPAHLFLDELGIEYDEQDNYVVIKHAALFTSTIMSKLLA 178
Query: 597 KPNVKLFNAVAAEDLIVKEGKVAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDG 776
+PNVKLFNAVAAEDLIVK G+V GVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCGHDG
Sbjct: 179 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 238
Query: 777 PFGATGVR 800
PFGATGV+
Sbjct: 239 PFGATGVK 246
Score = 32.3 bits (72), Expect(2) = 2e-94
Identities = 14/15 (93%), Positives = 15/15 (100%)
Frame = +1
Query: 811 IGMIDSVPGMKALDM 855
IGMID+VPGMKALDM
Sbjct: 251 IGMIDTVPGMKALDM 265
>sp|Q38814|THI4_ARATH Thiazole biosynthetic enzyme, chloroplastic
OS=Arabidopsis thaliana GN=THI1 PE=1 SV=1
Length = 349
Score = 332 bits (850), Expect(2) = 5e-93
Identities = 161/188 (85%), Positives = 174/188 (92%)
Frame = +3
Query: 237 FKFEPIKESIVSREMTRRYMMDMITYADTDXXXXGAGSAGLSCAYEMSKNPNINIAIIEQ 416
F F+PIKESIVSREMTRRYM DMITYA+TD GAGSAGLS AYE+SKNPN+ +AIIEQ
Sbjct: 56 FTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAIIEQ 115
Query: 417 SVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGVEYDEQDTYVVIKHAALFTSTIMSKLLA 596
SVSPGGGAWLGGQLFSAM+VRKPAH+FLDE+GV YDEQDTYVV+KHAALFTSTIMSKLLA
Sbjct: 116 SVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSKLLA 175
Query: 597 KPNVKLFNAVAAEDLIVKEGKVAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDG 776
+PNVKLFNAVAAEDLIVK +V GVVTNWALV+ NH TQSCMDPNVMEAKIVVSSCGHDG
Sbjct: 176 RPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCGHDG 235
Query: 777 PFGATGVR 800
PFGATGV+
Sbjct: 236 PFGATGVK 243
Score = 31.6 bits (70), Expect(2) = 5e-93
Identities = 14/15 (93%), Positives = 14/15 (93%)
Frame = +1
Query: 811 IGMIDSVPGMKALDM 855
IGMID VPGMKALDM
Sbjct: 248 IGMIDHVPGMKALDM 262
>sp|Q41738|THI41_MAIZE Thiazole biosynthetic enzyme 1-1,
chloroplastic OS=Zea mays GN=THI1-1 PE=2 SV=1
Length = 354
Score = 330 bits (846), Expect(2) = 4e-92
Identities = 160/188 (85%), Positives = 174/188 (92%)
Frame = +3
Query: 237 FKFEPIKESIVSREMTRRYMMDMITYADTDXXXXGAGSAGLSCAYEMSKNPNINIAIIEQ 416
F+F PIKESIVSREMTRRYM DMITYADTD GAGSAGLSCAYE+SK+P ++IAI+EQ
Sbjct: 62 FRFSPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKDPAVSIAIVEQ 121
Query: 417 SVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGVEYDEQDTYVVIKHAALFTSTIMSKLLA 596
SVSPGGGAWLGGQLFSAMVVRKPAH+FLDELGV YDE + YVVIKHAALFTST+MS LLA
Sbjct: 122 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDELGVAYDEAEDYVVIKHAALFTSTVMSLLLA 181
Query: 597 KPNVKLFNAVAAEDLIVKEGKVAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDG 776
+PNVKLFNAVA EDLIV+ G+V GVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCGHDG
Sbjct: 182 RPNVKLFNAVAVEDLIVRGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 241
Query: 777 PFGATGVR 800
PFGATGV+
Sbjct: 242 PFGATGVK 249
Score = 30.0 bits (66), Expect(2) = 4e-92
Identities = 13/15 (86%), Positives = 14/15 (93%)
Frame = +1
Query: 811 IGMIDSVPGMKALDM 855
IGMI +VPGMKALDM
Sbjct: 254 IGMISAVPGMKALDM 268
>sp|Q41739|THI42_MAIZE Thiazole biosynthetic enzyme 1-2,
chloroplastic OS=Zea mays GN=THI1-2 PE=2 SV=1
Length = 354
Score = 329 bits (843), Expect(2) = 1e-91
Identities = 156/188 (82%), Positives = 175/188 (93%)
Frame = +3
Query: 237 FKFEPIKESIVSREMTRRYMMDMITYADTDXXXXGAGSAGLSCAYEMSKNPNINIAIIEQ 416
F+F PIKESIVSREMTRRYM DMIT+ADTD GAGSAGLSCAYE+SK+P +++AI+EQ
Sbjct: 59 FRFSPIKESIVSREMTRRYMTDMITHADTDVVIVGAGSAGLSCAYELSKDPTVSVAIVEQ 118
Query: 417 SVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGVEYDEQDTYVVIKHAALFTSTIMSKLLA 596
SVSPGGGAWLGGQLFSAMVVR+PAH+FLDELGV YDE + YVV+KHAALFTST+MS+LLA
Sbjct: 119 SVSPGGGAWLGGQLFSAMVVRRPAHLFLDELGVGYDEAEDYVVVKHAALFTSTVMSRLLA 178
Query: 597 KPNVKLFNAVAAEDLIVKEGKVAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDG 776
+PNVKLFNAVA EDLIV+ G+V GVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCGHDG
Sbjct: 179 RPNVKLFNAVAVEDLIVRRGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 238
Query: 777 PFGATGVR 800
PFGATGV+
Sbjct: 239 PFGATGVK 246
Score = 30.0 bits (66), Expect(2) = 1e-91
Identities = 13/15 (86%), Positives = 14/15 (93%)
Frame = +1
Query: 811 IGMIDSVPGMKALDM 855
IGMI +VPGMKALDM
Sbjct: 251 IGMISAVPGMKALDM 265
>sp|P40998|THI2_SCHPO Thiazole biosynthetic enzyme, mitochondrial
OS=Schizosaccharomyces pombe (strain ATCC 38366 / 972)
GN=thi2 PE=2 SV=2
Length = 328
Score = 254 bits (649), Expect = 6e-67
Identities = 127/194 (65%), Positives = 154/194 (79%), Gaps = 6/194 (3%)
Frame = +3
Query: 237 FKFEPIKESIVSREMTRRYMMDMITYADTDXXXXGAGSAGLSCAYEM-SKNPNINIAIIE 413
F F PI+ES VSR MTRRY D+ YA++D GAGSAGL+ AY + ++ P++ IAIIE
Sbjct: 49 FSFAPIRESTVSRAMTRRYFSDLDKYAESDIVIVGAGSAGLTAAYYIGTRRPDLKIAIIE 108
Query: 414 QSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGVEYDEQDTYVVIKHAALFTSTIMSKLL 593
SV+PGGGAWLGGQLFSAMVVRKPA +FL+E+GV Y+++ YVV+KHAALFTST+M++ L
Sbjct: 109 ASVAPGGGAWLGGQLFSAMVVRKPADLFLNEIGVPYEDEGDYVVVKHAALFTSTVMARTL 168
Query: 594 AKPNVKLFNAVAAEDLIVKEGK-----VAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVS 758
A PNVKLFNA A EDLIVKEGK +AGVVTNW LVS+NH QSCMDPN + A +VVS
Sbjct: 169 ALPNVKLFNATAVEDLIVKEGKDGKQRIAGVVTNWTLVSLNHGLQSCMDPNTINAHLVVS 228
Query: 759 SCGHDGPFGATGVR 800
+ GHDGPFGA V+
Sbjct: 229 ATGHDGPFGAFCVK 242